WebJan 1, 2016 · For culturing non-neuronal cell lines, Dulbecco's modified Eagle's medium (DMEM, Sigma–Aldrich) and 10% fetal calf serum (FCS, Biowest) were used. HEK293 and NIH3T3 cells were maintained in DMEM supplemented with 10% FCS (Biowest S1820), 2% GlutaMAX, 50 U/ml penicillin and 50 μg/ml streptomycin (Sigma–Aldrich) at 37 °C in a … WebBioWest - Fetal Bovine Serum (USA Origin) S1520 Safety data sheet According to 1907/2006/EC (REACH), 453/2010/EU, 2015/830/EU SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING 1.4 Emergency telephone number: ORFILA: 01 45 42 59 59 Biowest SAS Rue de la Caille 49340 …
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WebJul 1, 2016 · Both cell lines were grown in Dulbecco's modified Eagle's medium (Invitrogen), supplemented with 10% fetal bovine serum (BioWest, S1820-500), 10 U/ml penicillin/streptomycin (Invitrogen). Human neurons (HN) were purchased from ScienCell Research Laboratories, cells were grown in Neuronal medium supplemented with … WebBiowest guarantees the best quality : We are ISO 9001 and ISO 13485 certified. Biowest is registered by the French Ministry of Agriculture (regulation (EC) n° 1069 / 2009) under the agreement n° FR 49 .231 .001 for the production of animal sera . Company profile Biowest, a real partner for 30 years in scientific breakthroughs... child\u0027s play franchise movies
Simple method for large-scale production of macrophage …
WebHuman embryonic kidney (HEK) 293T cells and MEFs were cultured in Dulbecco's Modified Eagle Medium (SIGMA, D5796) with fetal bovine serum (BioWest, S1820) and penicillin/streptomycin (Nacalai Tesque, 26253–84) at 37°C. WebOct 18, 2016 · This manuscript describes a protocol for the phenotypic and quantitative analysis of macrophages from rat kidneys by flow cytometry. This technique can be further followed by several assays, including cell sorting and mRNA or protein expression studies to allow in-depth characterization of the role of macrophages in renal disease. WebMar 11, 2024 · HT29 and HCT116 cells were cultured in Dulbecco's modified Eagle's medium (Sigma-Aldrich Corp, D5796) with 10% fetal bovine serum (Biowest, S1820) and 1% penicillin/streptomycin (Nacalai Tesque, 26253-84) at 37 °C. child\\u0027s play filmyzilla