Flp-intm t-rextm 293 细胞系

WebOct 25, 2024 · The Flp-InTM T-RExTM 293 cells were generated by cotransfection of the constructed plasmid and pOG44 plasmid to express the target gene. Successfully recombined monoclonal cell lines were screened ... WebOct 31, 2024 · HEK293细胞及其衍生株HEK293(human embryonic kidney 293),简称293细胞,来源于人胚胎肾细胞,由于在19号染色体整合了大约4.35 kb的腺病毒基因组片段,使其细胞周期以及细胞表型、核型等发生了 ... 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性(ecotropic)报告质粒和 ...

flp-in t-rex 293 Cells Thermo Fisher Scientific - JP

WebFigure 4-2. TUG-891 but not TUG-1197 displays low potency mediated activation of hFFA1. Flp-InTM T-RExTM 293 cells able to express hFFA1-mVenus-HA were seeded (75,000 cells /well) into poly-D-lysine coated black clear-bottom 96-well plates. Expression of hFFA1 was induced by the antibiotic doxycycline (100 ng/ml) for 18-24 hours. Cells were … WebFlp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression … Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes easter decorations with plastic eggs https://austexcommunity.com

Epigenetics in Cancer pdf epub mobi txt 电子书 下载 2024 - 静流 …

Web293FTM细胞则是来源于Flp-InTMT-RETM293细胞,该细胞系中转染了ecotropic receptor质粒及MAPPIT(mammalian protein-protein interaction trap)报告质粒,该细胞系主要用于 … WebT-REx™ 细胞系可稳定表达四环素抑制子蛋白(表 1)。使用 T-REx™ 系统可以节省大量的时间和精力。通过使用阳性对照载体 pcDNA™4⁄TO⁄lacZ 进行瞬时转染,对 T-REx™ 细胞系进行功能检测。在用四环素诱导时,T-REx™ 细胞系在抑制状态下表现出极低的基础表达水平,表达水平较高(图 1)。 WebApr 2, 2024 · 基本信息. 商品名称:Epigenetics in Cancer 作者:编者:JUNJIANG FU//SABER IMANI 定价:198 出版社:科学 ISBN号:9787030543769 其他参考信息 (以实物为准). 出版时间:2024-01-01 印刷时间:2024-01-01 版次:1 印次:1 开本:16开 包装:平装 页数:364 内容提要 easter decor at walmart

Figure 5-9 from Desensitisation of the human long chain fatty acid ...

Category:FLPINTREX293_价格-品牌-详情介绍_丁香通 - biomart.cn

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Flp-intm t-rextm 293 细胞系

FLPINTREX293_价格-品牌-详情介绍_丁香通 - biomart.cn

WebSep 29, 2024 · 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性(ecotropic)报告质粒和MAPPIT (mammalian protein-protein interaction trap) 报告质粒获得,常用于蛋白互作筛选。 293S 细胞. 293S细胞支持悬浮培养,S为Suspension悬浮的首字母,可以耐受低Ca2⁺培养体系。 Web丁香通为您提供FLPINTREX293商品详情介绍:价格:¥1 - 2000,货号:R78007,品牌:invitrogen,GIBCO,产地:美国,详见丁香通FLPINTREX293商品详情页;

Flp-intm t-rextm 293 细胞系

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Web技术资料. 规格:. 1ml. Flp-In™系统使您可以稳定整合和表达感兴趣的基因,以提供单拷贝等基因细胞系。. Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶标(FRT)位点。. 随后,包含您感兴趣的基因的表达载体会通过FRT位点的Flp重组酶介 … WebThe T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal …

WebJul 2, 2012 · Als Kontrollen dienten mit dem Leervektor transfizierte und hCtr1 überexprimierende Flp-InTM T-RExTM-293-Zellen. Die rekombinanten hCtr2-Zellen erwiesen sich als signifikant weniger sensitiv gegenüber Cis- und Carboplatin als die Kontrollzellen. hCtr2 hatte hier also einen resistenzfördernden Effekt. Dies ist ein …

WebMar 26, 2024 · Protocol 2 Protein complex purification from Flp-InTM T-RExTM 293 stably expressed FLAG tagged protein cell line Protocol 3 ChiP-Sequencing from Flp-InTM T-RExTM 293 stably expressed FLAG tagged protein cell lines Protocol 4 ChiP assay for endogenous gene Protocol 5 The cancer cell index, migration, and invasion assays Color … http://www.bio-thing.com/p118025

Web293t细胞由293细胞通过基因技术派生出的细胞系,是经过腺病毒e1a基因的转染,能表达sv40大t抗原,含有sv40复制起始点与启动子区。 许多真核表达载体如pcDNA3.1中含有SV40病毒的复制起始位点,可以在表达SV40病毒T抗原的细胞系中复制,从而提高外源基因 …

WebAug 5, 2024 · 293Flp-InTMT-RExTM Cells. 293Flp-In 293 T-REx细胞系是为了快速生成稳定的细胞系而设计的,它可以确保从Flp-In表达载体上获得的蛋白的均匀表达。这些细胞在转录活跃的基因组位点上包含一个稳定整合的FRT位点,有针对性的整合Flp-In表达载体,确保高水平目的基因。 cucumber sandwiches recipe with dillWebSep 29, 2024 · 293FTM 细胞 是由 Flp-InTM T-RExTM 293 ... 总之, 293系列细胞经过长时间的改造与优化,已在多个领域实现了较多的应用,源井生物对于293与293T细胞也有丰富的基因编辑和细胞模型构建的经验,并拥有上千种293细胞的KO现货,覆盖超20种信号通 … cucumber sandwiches on party ryeWebFlp-In™系统使您可以稳定整合和表达感兴趣的基因,以提供单拷贝等基因细胞系。Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶标(FRT)位点。随后,包含您感兴趣的基因的表达载体会通过FRT位点的Flp重组酶介导DNA重组,整合入基因组。 easter decor for church ideasWebThermo Fisher flp in t rex human embryonic kidney 293 hek293t cell line Flp In T Rex Human Embryonic Kidney 293 Hek293t Cell Line, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ... HEK293T-Flp-InTM T-RexTM (HEK293T) cells were maintained and cultured in DMEM media … easter decorations with lightsWebFeb 13, 2024 · flp/frt 系统就属于位点特异性重组系统的一种。 FLP是一个重组酶(flippase,Flp),类似于Cre,它识别的位点是FRT,当FRT在同一条DNA上且方向相 … easter decor for homeWebFlp-In TM T-REx TM 系统将Flp-In TM 系统的靶向整合与T-REx TM 系统的强大诱导表达能力整合在一起。该系统能够生成同基因,可诱导的稳定表达细胞系,并能够针对这些细 … easter decor for tiered trayWebHuman. The Flp-In T-REx-293 cell line stably expresses the lacZ-Zeocin fusion gene and the Tet repressor, and is designed for use with the Flp-In T-REx System. The cell line expresses the Tet repressor from pcDNA6/TR and contains a single integrated Flp Recombination Target (FRT) site from pFRT/lacZeo as confirmed by Southern blot analysis. easter decoration with balloon