Lysosensor yellow/blue dnd 160
Web22 oct. 2024 · Similar to the commonly used Lysotracker reagent, LysoSensor Yellow/Blue DND-160 is an acidotropic probe that accumulates in acidic lysosomes following … WebEnter the email address you signed up with and we'll email you a reset link.
Lysosensor yellow/blue dnd 160
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Web20 feb. 2015 · The chapter also includes references on the use, properties, and safety/toxicity of acridine homodimer on humans, animals and the environment. It presents a list of imaging/labeling applications, biological/medical applications and industrial applications of lysosensor yellow/blue DND 160. WebLysoSensor Yellow/Blue dextran is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. This dextran conjugate is taken up by the …
Web15 ian. 2009 · In contrast, excited state emission from LysoSensor Yellow/Blue DND-160, one of the few commercially available dual-wavelength dyes for sensing acidic pH, involves a “push–pull”-type intramolecular charge transfer (ICT) resulting in solvatochromic behavior . Given its solvent sensitivity, we investigate whether DND-160 can be used for ... WebLysoSensor™ Yellow/Blue DND-160 is a ratiometric probe that can be used to measure the pH of acidic organelles, such as lysosomes. The LysoSensor dye produces Invitrogen …
Web19 mar. 2003 · LysoSensor Yellow/Blue DND-160 (DND-160) is a membrane-permeable probe and can rapidly highlight the acidic compartments within 5 min. Nevertheless, DND-160 also leaked out the cells in a short time and quickly increased the image background. Web1 nov. 2008 · LysoSensor Yellow/Blue DND-160, a dual-wavelength fluorophore commonly used for sensing pH in acidic organelles, possesses solvatochromic behavior …
WebThe ratiometric fluorescent dye LysoSensor™ Yellow/Blue DND-160 (cat # L7545, Thermo Fisher Scientific) was used to measure the pH of lysosomes. This dye permeates live cells and accumulates in the lysosomes where it shifts fluorescence from blue to yellow depending on the acidity.
Web15 ian. 2009 · LysoSensor Yellow/Blue DND-160, a dual-wavelength fluorophore commonly used for sensing pH in acidic organelles, possesses solvatochromic behavior believed to originate from an intramolecular charge transfer (ICT). Given this, we investigated whether DND-160 can be used for acidic pH sensing under hydrostatic … cherine facebookWebМосковская обл., г. Пущино, мкр. [email protected]. 8 (495)-790-33-63 cherine cohenWeb1 iun. 2013 · Lysosensor Yellow/Blue DND-160 (1 μm) was added for 5 minutes. Alexafluor 546-Dextran (10 μg/ml) was added for 12 hrs with additional 6 hr chase prior to the addition of Lysosensor DN-160. Cells were washed 3 times with HBSS then imaged in dye free media with plan-NeoFluor 100X/1.3 oil DIC objective lens on a Zeiss Axiovert … flights from honolulu to newark airportWeb1 mai 2024 · The LysoSensor™ Yellow/Blue DND-160 Kit (Yeasen) was used according to the manufacturer’s protocol. Briefly, cells were seeded on coverslips in a 24-well plate and treated with NaF for 24 h, and loaded with 4 μmol/L LysoSensor Yellow/Blue DND-160 of DMEM for 15–30 min at 37 ℃. Then, the cells were washed 3 times with PBS buffer and ... cherine fahmyWeb1 nov. 2008 · LyoSensor Yellow/Blue DND 160 is a ratiometric probe that produces blue fluorescence in neutral environments and yellow fluorescence in more acidic environments. 21 Using this staining,... flights from honolulu to mspWebLysoSensor Yellow/Blue 10,000 MW dextran. 381. 335. 521 †. 452 ‡. 3.9. * Absorption (Abs) and fluorescence emission (Em) maxima, determined in aqueous buffer or methanol; values may vary somewhat in cellular environments. † At pH 3. ‡ At pH 7; this dye has pH-dependent dual-excitation and dual-emission peaks. cherine fok iiaWeb15 ian. 2009 · LysoSensor Yellow/Blue DND-160, a dual-wavelength fluorophore commonly used for sensing pH in acidic organelles, possesses solvatochromic behavior … cherine energy stock