Phospho- ser/thr pka substrate antibody
WebIn addition, a phospho- (Ser/Thr) PKA substrate antibody detected bands on immunoblot analysis of trans-sialidase antibody precipitated proteins from parasite lysate and the … WebImmunogen was a synthetic phospho-PKA substrate peptide. Phospho- (Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine …
Phospho- ser/thr pka substrate antibody
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WebA pan-SIK–specific kinase inhibitor reduces both the hyperphosphostate and the elevated sleep need in Sleepy mutant mice, as well as sleep-deprived WT mice. SIK3 has a serine-threonine kinase domain at the N terminus and a protein kinase A (PKA) recognition site S551 in the middle portion ( 7 ). WebPhospho- (Ser/Thr) PKA Substrate Antibody. *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca.
WebSep 9, 2005 · Western blot using a phospho-Thr antibody of GST-EEA1 (1257–1411) wt or T1392A (1 μg) after phosphorylation with MKK6DD-activated p38α or MKK6DD alone (right panels). ( B ) Endogenous EEA1 was immunoprecipitated from MKK6DD-expressing HEK293 cells (left panel) or SH-SY5Y cells (right panel) and blotted with antibodies against EEA1 …
WebImmunodetection of phospho- (Ser/Thr) PKA substrates shows that phosphorylated proteins are localized differently in spermatozoa of carp and sterlet. Strong phosphorylation of PKC substrate was observed in flagella of sterlet spermatozoa, whereas in carp sperm, PKC substrates were lightly phosphorylated in the midpiece and flagella. WebFor antiphospho-PKA substrate (RRXS/T) (100G7E) antibody, a rabbit monoclonal antibody against pPKA substrate was used (commercially available from Cell Signaling). For …
Webdetermining its substrate specificity, we assayed human recombi-nant AurA on a variety of phosphoacceptor peptide substrates in-cluding a series of properly modified derivatives of the Kemptide (ALRRASLGAA). The data presented here show that AurA is a basophilic Ser/Thr protein kinase recognizing the consensus
WebThe NeuroMab™ anti-Phospho-PKA C (Thr197) antibody detects endogenous levels of PKA C (-α, -β, and -γ) only when phosphorylated at Thr197. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr197 of human PKA C protein. Store at -20°C. Do not aliquot the antibody. sign in cox internetWebThe Anti-phospho PKA-Eu cryptate is a labeled polyclonal antibody enabling the detection of peptides and proteins containing a phosphorylated Ser/Thr residue with Arginine in the 3 position. It does not detect the non-phosphorylated PKA. This allows the construction of a highly specific biochemical kinase assay. the pursuit of status in social groupsWebPhospho-CDK9 (Thr186) Antibody detects endogenous levels of CDK9 only when phosphorylated at Thr186. ... Thr-350, Ser-353, Thr-354 and Ser-357 triggers kinase activity by promoting cyclin and substrate binding (e.g. HIV TAT) upon conformational changes. Thr-186 phosphorylation requires the calcium Ca(2+) signaling pathway, including CaMK1D … sign in craigslistWebSee all Phospho - (Ser/Thr) Phe primary antibodies Description Rabbit polyclonal to Phospho - (Ser/Thr) Phe Host species Rabbit Tested applications Suitable for: ELISA, IP, WB, ICC/IF more details Species reactivity Reacts with: Species independent Immunogen Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . sign in coxWebProtein kinase A (PKA), also known as cAMP-dependent protein kinase (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of … sign in craWebPhospho-(Ser/Thr) AMPK Substrate (P-S/T2-102) Rabbit mAb preferentially recognizes endogenous proteins and peptides bearing the LXRXXpS/pT motif. The antibody also … the pursuit of possibilityWebJun 25, 2015 · A phospho-(Ser/Thr) PKA substrate antibody (Cell Signaling Technology) was used to detect in vivo phosphorylation of CP. Constructions of plasmids. sign in cra business